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KMID : 0895820150250020051
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Journal of Oriental Rehabilitation Medicine
2015 Volume.25 No. 2 p.51 ~ p.64
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Effects of Gyejigabuja-tang on MIA-induced Osteoarthritis in Rats
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Won Je-Hoon
Woo Chang-Hoon
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Abstract
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Objectives : This study was carried out to investigate the effects of Gyejigabuja-tang extracts on the Monosodium iodoacetate (MIA) induced osteoarthritis in rats.
Methods : Osteoarthritis was induced by injection of MIA into knee joint cavity of rats. Rats are divided into 4 groups (normal, control, positive comparison group, GBT group, each n=5). Normal group was injected by normal saline into knee joint cavity only. Control group was induced for osteoarthritis by MIA and oral medicated with distilled water. Positive comparison group was injected with MIA and taken Joins tablet 25 mg/kg. GBT group was injected with MIA and taken Gyejigabuja-tang extracts 300 mg/kg. Positive comparison group and GBT group were oral medicated for each substance once a day for 4 weeks. ALT, AST and creatinine were evaluated for hepatotoxicity and nephrotoxicity. Hind paw weight bearing ability was examined and inflammatory cytokines (IL-1β, IL-6, TNF-α), PGE2, LTB4, osteocalcin and deoxypyridinoline (DPD) within serum were analysed. Knee joint structures were observed by Hematoxylin & Eosin (H&E), Safranin-O staining method.
Results : 1. Function of liver and kidney was not affected. 2. Hind paw weight bearing ability was significantly improved. 3. IL-1β, IL-6 and TNF-α in experimental group were significantly decreased compared with control group. 4. PGE2, LTB4, Osteocalcin and DPD in experimental group were decreased compared with control group. 5. In histopathologic observation, injury on synovial membrane and cartilage of experimental group was lesser than control group (H&E, Safranin-O staining).
Conclusions : Based on these results, it can be suggested that Gyejigabuja-tang has anti-inflammation effects on the MIA-induced osteoarthritis in rats.
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KEYWORD
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Gyejigabuja-tang, Osteoarthritis, Anti-inflammation effect, Monosodium iodoacetate, MIA
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